Journal: Cell stem cell

Article Title: A drug screen using human iPSC-derived hepatocyte-like cells identifies cardiac glycosides as a potential treatment for hypercholesterolemia

doi: 10.1016/j.stem.2017.01.011

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: A sandwich ELISA to detect human albumin in tissue culture supernatants and mouse sera used a 1/100 dilution of a capture human albumin coating antibody (Bethyl laboratories, A80-129A) and a 1:85,000 dilution of a Horseradish Peroxidase (HRP) conjugated human albumin detection antibody (Bethyl laboratories, A80-129P).

Techniques: Recombinant, Purification, Reverse Transcription, Gene Expression, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, In Vitro, Sequencing, Software

Journal: Frontiers in Veterinary Science

Article Title: Intramammary Immunisation Provides Short Term Protection Against Mannheimia haemolytica Mastitis in Sheep

doi: 10.3389/fvets.2021.659803

Figure Lengend Snippet: Details of antibodies used for cytokine ELISAs.

Article Snippet: Bovine IL-17A Coating , Polyclonal , n/a, rabbit , 2 , Kingfisher Biotech.

Techniques:

Journal: Cells

Article Title: A Novel Small-Molecule Inhibitor of Endosomal TLRs Reduces Inflammation and Alleviates Autoimmune Disease Symptoms in Murine Models

doi: 10.3390/cells9071648

Figure Lengend Snippet: Cell viability measurement and TNF-α-inhibitory profile of TAC5. ( A ) RAW 264.7 cells were treated with selected compounds at different concentrations (1, 10, 50, or 100 µM) for 24 h. Cell viability was measured by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The percentage of viable cells as compared to control is presented in a histogram. All experiments were independently conducted three times and mean ± SEM of the independent experiments was calculated by a two-tailed paired Student’s t -test (* p < 0.05, ** p < 0.01). ( B ) RAW 264.7 cells were treated with the compounds TAC2, TAC5, and from TAC8 to TAC11 at concentrations of 12.5, 25, and 50 µM in the presence and/or absence of CL075 for 24 h. The secreted levels of TNF-α were measured in the cell culture supernatant by a TNF-α enzyme-linked immunosorbent assay (ELISA) kit. ( C ) A two-dimensional representation of the chemical structure of TAC5. All experiments were independently conducted five times and the mean ± SEM of the independent experiments was calculated by a two-tailed paired Student’s t -test (* p < 0.05, ** p < 0.01).

Article Snippet: Specifically, mouse urinary albumin levels were analyzed with a mouse albumin ELISA kit (41-ALBMS-E01, Alpco Diagnostics, Salem, NH, USA) according to the manufacturer’s protocol.

Techniques: MTT Assay, Control, Two Tailed Test, Cell Culture, Enzyme-linked Immunosorbent Assay